Genome-editing techniques are promising tools in plant breeding. To facilitate a more comprehensive understanding of the current and future applications of genome editing in crops, EU-SAGE developed an interactive, publicly accessible online database of genome-edited crops.

The aim of the database is to inform interested stakeholder communities in a transparent manner about the latest evidence about genome editing applications in crops. Different elements including the plant species, traits, techniques, and applications can be filtered in this database.

Regarding the methodology, a literature search in the bibliographic databases and web pages of governmental agencies was conducted using predefined queries in English. Identifying research articles in other languages was not possible due to language barriers. Patents were not screened.

Peer-reviewed articles were screened for relevance and were included in the database based on pre-defined criteria. The main criterium is that the research article should describe a research study of any crop developed for market-oriented agricultural production as a result of a genome editing.

This database will be regularly updated. Please contact us via the following webpage (https://www.eu-sage.eu/contact) in case you would like to inform us about a new scientific study of crops developed for market-oriented agricultural production as a result of genome editing.

This work has been supported by Task Force Planet Re-Imagine Europa (https://reimagine-europa.eu/area/planet)

Plant

Displaying 182 results

Traits related to abiotic stress tolerance

Enhanced responses to abscisic acid (ABA), which plays an important role in drought stress responses in plants. Improved drought tolerance through stomatal regulation and increased primary root growth under non-stressed conditions.
( Ogata et al., 2020 )
SDN1
CRISPR/Cas
Japan International Research Center for Agricultural Sciences (JIRCAS)
RIKEN Center for Sustainable Resource Science
University of Tsukuba, Japan
Enhanced resistance to salt and oxidative stress and increased grain yield.
( Alfatih et al., 2020 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences, China
Drought tolerance and abscisic acid sensitivity.
( Lou et al., 2017 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences, China
Potassium deficiency tolerance and contribution to stomatal closure.
( Mao et al., 2016 )
SDN1
CRISPR/Cas
Fujian Agriculture and Forestry University
Fujian Academy of Agricultural Sciences
National Center of Rice Improvement of China
National Engineering Laboratory of Rice
South Base of National Key Laboratory of Hybrid Rice of China, China
Salt tolerance.
( Duan et al,. 2016 )
SDN1
CRISPR/Cas
Anhui Academy of Agricultural Sciences, China
Arsenic (As) tolerance. As is toxic to organisms and elevated As accumulation may pose health risks to humans.
( Duan et al., 2015 )
SDN1
CRISPR/Cas
Anhui Academy of Agricultural Sciences, China
Improved yield and cold tolerance. High yield and high cold tolerance were often antagonistic to each other.
( Zeng et al., 2020 )
SDN1
CRISPR/Cas
College of Life Sciences, Wuhan University, China
Curled leaf phenotype and improved drought tolerance.
( Liao et al., 2019 )
SDN1
CRISPR/Cas
Guangxi University
South China Agricultural University, China
Enhanced salinity tolerance.
( Zhang et al., 2019 )
SDN1
CRISPR/Cas
Huazhong Agricultural University
Shanghai Agrobiological Gene Center, China
Enhanced the tolerance of plants to salt (NaCl), the stress hormone abscisic acid (ABA), dehydration and polyethylene glycol (PEG) stresses.
( Yue et al., 2020 )
SDN1
CRISPR/Cas
Zhejiang University
Hunan Agricultural University, China
Salinity tolerance. Salinity stress is one of the most important abiotic stress factors affecting rice production worldwide.
( Lim et al., 2021 )
SDN1
CRISPR/Cas
Kangwon National University
Sangji University
Kyung Hee University, South Korea
Drought tolerance by modulating lignin accumulation in roots.
( Bang et al, 2021 )
SDN1
CRISPR/Cas
Seoul National University, South Korea
Enhances adaptation to direct-seeding on wet land and tolerance to drought stress in rice. Water stress is the most important factor limiting rice agriculture by either floods or drought.
( Zhang et al., 2020 )
SDN1
CRISPR/Cas
Huazhong Agricultural University
Shanghai Agrobiological Gene Center, China
Increased tolerance to salinity stress. Improved rice yields in saline paddy fields by root angle modifications to adapt to climate change.
( Kitomi et al., 2020 )
SDN1
CRISPR/Cas
National Agriculture and Food Research Organization (NARO)
Tohoku University
Institute of Agrobiological Sciences
Japan Science and Technology Agency (JST)
Advanced Analysis Center
National Institute of Advanced Industrial Science and Technology (AIST), Japan
Salt tolerance during the seedling stage.
( Chen et al., 2022 )
SDN1
CRISPR/Cas
Hubei Academy of Agricultural Sciences
Huazhong Agriculture University
Hubei Hongshan Laboratory, China
Drought tolerance.
( Zhao et al., 2022 )
SDN1
CRISPR/Cas
Hebei Normal University
University of Chinese Academy of Sciences, China
Improved salt stress resistance. Significant increase in the shoot weight, the total chlorophyll content, and the chlorophyll fluorescence under salt stress. Also high antioxidant activities coincided with less reactive oxygen species (ROS).
( Shah Alam et al., 2022 )
SDN1
CRISPR/Cas
Zhejiang University, China
Taif University, Saudi Arabia
Alexandria University, Egypt

Traits related to improved food/feed quality

Reduction of amylose content (AC). AC is the predominant factor determining rice eating and cooking quality.
( He et al., 2020 )
SDN1
CRISPR/Cas
Northeast Agricultural University
Chinese Academy of Sciences
Jiangsu Academy of Agricultural Sciences
Northeast Agricultural University, China
Reduction in cadmium accumulation. Cadmium is a heavy metal, harmful for human health. Cadmium accumulation represents a severe threat to people consuming rice as a staple food.
( Yang et al., 2019 )
SDN1
CRISPR/Cas
Nanjing Agricultural University
Chinese Academy of Sciences, China
Improved aleurone layer with enhanced grain protein content. Improved grain nutritional quality by improved accumulation of essential dietary minerals (Fe, Zn, K, P, Ca) in the endosperm of rice grain. Improved root and shoot architecture.
( Achary et al., 2021 )
SDN1
CRISPR/Cas
International Centre for Genetic Engineering and Biotechnology, India
Generation of a new glutinous Photothermosensitive Genic-Male-Sterile (PTGMS) line with a low amylose content. PTMGS line combines high-quality and high-light-efficiency use, disease and stress resistance.
( Teng et al., 2021 )
SDN1
CRISPR/Cas
Guangxi University
South China Agricultural University, China
High-quality sugar production by rice (98% sucrose content). Carbohydrates are an essential energy-source. Sugarcane and sugar beet were the only two crop plants used to produce sugar.
( Honma et al., 2020 )
SDN1
CRISPR/Cas
Fujian Agriculture and Forestry University, China
Faculty of Engineering
Kitami Institute of Technology
NagoyaUniversity
Tokyo Metropolitan University, Japan
Carnegie Institution for Science, USA
Reduce malnutrition by decreasing antinutrient phytic acid (PA) and increasing Iron and Zinc accumulation. PA has adverse effects on essential mineral absorption and thus is considered as an anti-nutritive for monogastric animals.
( Ibrahim et al., 2021 )
SDN1
CRISPR/Cas
Quaid-i-Azam University Islamabad
National Agricultural Research Centre, Pakistan
Production of high amylose and resistant starch rice. Starch accounts for 80 to 90% of the total mass of rice seeds and is low in resistant starch (RS), which is beneficial in preventing various diseases. Starch with high amylose content (AC) and RS have a lower GI value. Foods with low GI value have beneficial effects on glycemic control.
( Wang et al., 2021 )
SDN1
CRISPR/Cas
National Chiayi University
Taiwan Agricultural Research Institute Chiayi Agricultural Experiment Branch, Taiwan
Fragrance by accumulation of the natural aroma substance 2-acetyl-1-pyrroline (2AP). Fragrance is one of the most important rice quality traits, with 2AP being the major contributor to aroma.
( Tang et al., 2021 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences
Hubei Academy of Agriculture Sciences
Guangdong Academy of Agricultural Sciences, China
Agricultural Research Center, Egypt
Improved amylose levels to influence grain eating and cooking quality (ECQ).
( Huang et al., 2020 )
SDN1
CRISPR/Cas
Yangzhou University, China
Improve glutinosity in elite varieties. Decreased amylose content without affecting other desirable agronomic traits.
( Zhang et al., 2018 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences, China
Purdue University
University of Queensland, USA
Fragrant rice.
( Shan et al., 2015 )
SDN1
TALENs
Chinese Academy of Sciences, China
Increased amylose content. Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits and reduce risks of diseases such as coronary heart disease, diabetes and certain colon and rectum cancers.
( Sun et al., 2017 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences, China
University of California, USA
University of Liege, Belgium
Reduced arsenic content, a highly toxic metalloid harming human health. Inorganic Arsenic is listed as a carcinogen.
( Ye et al., 2017 )
SDN1
CRISPR/Cas
Huazhong Agricultural University, China
Altered fatty acid composition. High oleic/low linoleic acid rice. Oleic acid has potential health benefits and helps decrease lifestyle disease.
( Abe et al., 2018 )
SDN1
CRISPR/Cas
National Agriculture and Food Research Organization, Japan
Reduced cesium content. The production of radiocesium in food in contaminated soils is a serious health concern.
( Nieves-Cordones et al., 2017 )
SDN1
CRISPR/Cas
Université Montpellier, France
Reduced cadmium content. Cadmium poses a health treath, as it is a highly toxic heavy metal for most living organisms.
( Tang et al., 2017 )
SDN1
CRISPR/Cas
Hunan Agricultural University, Hunan Hybrid Rice Research Center, Normal University, China
Carotenoid accumulation to solve the problem of vitamin A deficiency that is prevalent in developing countries.
( Endo et al., 2019 )
SDN1
CRISPR/Cas
National Agriculture and Food Research Organization
Ishikawa Prefectural University, Japan
Fine-tuning the amylose content, one of the major contributors to the eating and cooking quality.
( Xu et al., 2021 )

BE
Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement
Yangzhou University
Chinese Academy of Sciences, China
CSIRO Agriculture and Food, Australia
Reduction of phytic acid (PA) in seeds. PA has adverse effects on essential mineral absorption and thus is considered as an anti-nutritive for monogastric animals.
( Khan et al., 2019 )
SDN1
CRISPR/Cas
Zhejiang University
Yangtze University, China
Improved grain quality. The amylose content, gel consistency and pasting viscosity of grain starches are influencing the grain appearance, cooking/eating quality and starch physical characters.
( Zeng et al., 2020 )
SDN1
CRISPR/Cas
State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources
Guangdong Laboratory for Lingnan Modern Agriculture
South China Agricultural University, China
Improved quality by reduced grain protein content (GPC). High GPC is negatively correlated between protein content and peak viscosity and breakdown value. High GPC is also positively correlated to protein content and hardness.
( Wang et al., 2020 )
SDN1
CRISPR/Cas
Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops
Agricultural College of Yangzhou University, China
Production of opaque seeds with depleted starch reserves. Reduced starch content and increased amylose content. Accumulation of multiple sugars, fatty acids, amino acids and phytosterols.
( Baysal et al., 2020 )
SDN1
CRISPR/Cas
University of Lleida-Agrotecnio Center
Catalan Institute for Research and Advanced Studies (ICREA), Spain
Royal Holloway University of London, UK
Carotenoid-enriched. Carotenoids, the source of pro vitamin A, are an essential component of dietary antioxidants.
( Dong et al., 2020 )
SDN3
CRISPR/Cas
University of California
Innovative Genomics Institute
The Joint Bioenergy Institute, USA
Fragrant rice. Introduction of aroma into any non-aromatic rice varieties.
( Ashokkumar et al., 2020 )
SDN1
CRISPR/Cas
Tamil Nadu Agricultural University, India
Increased lysophospholipid content and enhanced cooking and eating quality. Lysophospholipid (LPL) is derived from the hydrolysis of phospholipids and plays an important role in rice grain quality.
( Khan et al., 2020 )
SDN1
CRISPR/Cas
Zhejiang University, China
High gamma-aminobutyric acid (GABA) content. GABA plays a key role in plant stress responses, growth, development and as a nutritional component of grain can also reduce the likelihood of hypertension and diabetes. Increased amino acid content. Higher seed weight and seed protein content.
( Akama et al., 2020 )
SDN1
CRISPR/Cas
Shimane University
Institute of Agrobiological Sciences
National Agriculture and Food Research Organization
Yokohama City University, Japan
Increased flavonoid content, functioning as allelochemicals and insect deterrents.
( Lam et al., 2019 )
SDN1
CRISPR/Cas
The University of Hong Kong
The Chinese University of Hong Kong
Shenzhen
Zhejiang Academy of Agricultural Sciences
Nanjing Forestry University, China
Kyoto University, Japan
Low Cadmium (Cd) accumulating. Cadmium (Cd) is a non-essential heavy metal that is toxic to virtually all living organisms, including plants.
( Songmei et al., 2019 )
SDN1
CRISPR/Cas
Zhejiang University
Hubei Collaborative Innovation Center for Grain Industry
Zhejiang University
Jiaxing Academy of Agricultural Sciences, China
Increased carotene accumulation in rice endosperm.
( Shao et al., 2017 )
SDN1
CRISPR/Cas
Key Laboratory of Rice Biology and Genetic Breeding, China
Biofortification: Enhanced Zinc and Manganese tolerance and increased Zinc and Manganese accumulation in rice grains.
(Qiao et al., 2019)
SDN1
CRISPR/Cas
Shenzhen University
University of Chinese Academy of Sciences, China
Aromatic three-line hybrid.
( Hui et al., 2021 )
SDN1
CRISPR/Cas
China National Rice Research Institute, China
Increased grain amylose content. Improving grain quality is one of the most important goals in rice breeding. Contribute to the breeding of rice cultivars with better eating and cooking quality, as cooking and eating quality is determined from amylose content.
( Liu et al., 2022 )
SDN1
CRISPR/Cas
Hunan Agricultural University
China National Seed Group Co., China
Low amylose content to improve the rice eating quality.
( Mao et al., 2022 )

Guangdong Academy of Agricultural Sciences
Guangdong Key Laboratory of New Technology in Rice Breeding
Guangdong Rice Engineering Laboratory, China
Increased grain weight and grain size. Carbohydrate and total protein levels also increased.
( Guo et al., 2021 )
SDN1
CRISPR/Cas
Sichuan Agricultural University, China
University of California, USA
Facilitated Isoproturon Metabolism and Detoxification: Improved growth, the Isoproturon (IPU)-induced cellular damage was attenuated, and IPU accumulation was significantly repressed
(Zhai et al., 2022)
SDN1
CRISPR/Cas
Nanjing Agricultural University, China
Increased RS. Cereals high in RS may be beneficial to improve human health and reduce the risk of diet-related chronic diseases.
( Biswas et al., 2022 )
SDN1
CRISPR/Cas
Texas A&
M Univ.
Avance Biosciences Inc., USA
Reduced Cd accumulation.
( Chen et al., 2022 )
SDN1
CRISPR/Cas
South China Agricultural University
Guangdong Academy of Sciences, China
Imrpoved rice eating and cooking quality with down-regulated rice grain protein content, which is negatively regulated to ECQ.
( Yang et al., 2022 )
SDN1
CRISPR/Cas
Yangzhou University, China

Traits related to increased plant yield and growth

Improvement of yield by reducing the "easy to shatter" trait. Reduced seed shattering ensures better stability during the harvesting processes and improved yields.
( Sheng et al., 2020 )
SDN1
CRISPR/Cas
Hunan Agricultural University
Hunan Hybrid Rice Research Center
Hunan Academy of Agricultural Sciences, China
Increased yield under different environmental conditions: well-watered, drought, normal nitrogen and low nitrogen field conditions and at multiple geographical locations.
(Wang et al., 2020)
SDN1
CRISPR/Cas
Sinobioway Bio-Agriculture Group Co.
Ltd
Corteva Agriscience
Johnston, USA
Improved rice photosynthetic efficiency and yield: increased light saturation points, stomatal conductance, light tolerance and photosynthetic yields.
(Ye et al., 2021)
SDN1
CRISPR/Cas
South China Agricultural University, China
Semi-dwarf phenotype to improve product and lodging resistance.
( Zhang et al., 2020 )
SDN1
CRISPR/Cas
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, China
Control grain size and seed coat color.
( Tra et al., 2021 )

BE
International Rice Research Institute, Philippines
Dahlem Center of Plant Sciences Freie Universität, Germany
Synthetic Biology, Biofuel and Genome Editing R&
D Reliance Industries Ltd, India
Increased yield potential by nitrogen use efficiency. Nitrogen fertilizer has been applied broadly to increase yield. However, low nitrogen use efficiency causes environmental pollution and ecological deterioration by the nitrogen fertilizers.
( Zhang et al., 2021 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences
Zhengzhou University, China
Improved grain yield by modulating pyruvate enzymes and cell cycle proteins, leading to increased grain size. The grain size is a major determinant for rice yield and a vital trait for domestication and breeding.
( Usman et al., 2020 )
SDN1
CRISPR/Cas
Guangxi University
South China Agricultural University, China
Improved yield and fragrance.
( Usman et al., 2020 )
SDN1
CRISPR/Cas
Guangxi University
South China Agricultural University, China
Early flowering and maturity. Flowering time (heading date) is an important trait for crop yield and cultivation.
( Wang et al., 2020 )
SDN1
CRISPR/Cas
Sinobioway Bio-Agriculture Group, Co., China
Corteva™ Agriscience, USA
Plant architecture: high tillering and reduced height.
(Butt et al., 2018)
SDN1
CRISPR/Cas
King Abdullah University of Science and Technology, Saudi Arabia
Improved nitrogen use efficiency.
( Li et al., 2018 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences
Huazhong Agricultural University, China
University of California, USA
Improvement of grain weight. Longer panicle.
( Xu et al., 2016 )
SDN1
CRISPR/Cas
China National Rice Research Institute, China
China Three Gorges University, China
Altered grain number per panicle and increased seed weight.
( Li et al., 2016 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences, China
Altered grain number per panicle.
( Shen et al., 2016 )
SDN1
CRISPR/Cas
National Rice Research Institute, China
Increased seed weight.
( Hu et al., 2018 )
SDN1
CRISPR/Cas
Fudan University, China
Increased seed weight.
( Shen et al., 2017 )
SDN1
CRISPR/Cas
Yangzhou University, China
Increased seed weight.
( Ji et al., 2017 )
SDN1
CRISPR/Cas
Agronomy College of Henan Agricultural University, China
Genetic diversity.
( Shen et al., 2017 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences, China
Yangzhou University, China
Promote outgrowth buds and increase tiller number.
( Lu et al., 2017 )
SDN1
CRISPR/Cas
Wuhan Institute of Bioengineering
Huazhong Agricultural University
Chinese Academy of Sciences, China
Generating male sterility lines (MLS). Using MLS in hybrid seed production for monoclinous crops reduces costs and ensures high purity of the varieties because it does not produce pollen and has exserted stigmas. Complete abolition of pollen development.
( Lee et al., 2016 )
SDN1
CRISPR/Cas
Kyung Hee University, South Korea
Generating male sterility lines (MLS). Using MLS in hybrid seed production for monoclinous crops reduces costs and ensures high purity of the varieties because it does not produce pollen and has exserted stigmas.
( Li et al., 2016 )
SDN1
CRISPR/Cas
Shanghai Jiao Tong University, China
Generating male sterility lines (MLS). Using MLS in hybrid seed production for monoclinous crops reduces costs and ensures high purity of the varieties because it does not produce pollen and has exserted stigmas.
( Xie et al., 2017 )
SDN1
CRISPR/Cas
South China Agricultural University, China
Regulation of pollen tube growth. The tube grows in female reproductive tissues to transport two sperm cells into the embryo sac for double fertilization during sexual reproduction.
( Liu et al., 2017 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences
University of Chinese Academy of Sciences, China
Increased grain number per main panicle and an increased seed settling rate.
( Qian et al., 2017 )
SDN1
CRISPR/Cas
China Agricultural University, China
Grain yield, regulation of seed development.
( Yuan et al., 2017 )
SDN1
CRISPR/Cas
Nanjing Agricultural University, China
Generation of important yield-related trait characteristics: dense and erect panicles and reduced plant height.
(Wang et al., 2017)
SDN1
CRISPR/Cas
Syngenta Biotechnology, China
Reduced seed dormancy: rapid and uniform germination of seeds is important for rice production. Mutant seeds began to germinate 1 day after sowing, while WT seeds needed 2 days.
(Jung et al., 2019)
SDN1
CRISPR/Cas
Hankyong National University
Chungbuk National University
Hanyang University, China
Central Luzon State University, Philippines
Plants with longer primary roots and more crown roots, as well as increased sensitivity to auxins and cytokinins. The rice root system is important for growth.
( Mao et al., 2019 )
SDN1
CRISPR/Cas
Fudan University
Sichuan Agricultural University
Shanghai Normal University
Chinese Academy of Sciences, China
Semi-dwarf phenotype. Plant height is an important agronomic trait of rice, it directly affects the yield potential and lodging resistance.
( Han et al., 2019 )
SDN1
CRISPR/Cas
Guangxi University
South China Agricultural University
Guangxi University, China
Semi-dwarf phenotype with desired agronomic traits: tolerance to low phosporus levels and broad-spectrum resistance to diseases and insects.
(Hu et al., 2019)
SDN1
CRISPR/Cas
China National Rice Research Institute, China
Range of beneficial phenotypes: additional tillers and smaller culms and panicles.
(Cui et al., 2020)
SDN1
CRISPR/Cas
China National Rice Research Institute
Huazhong Agricultural University, China
Yangzhou University, Nagoya University, Japan
Improved rice grain shape and appearance quality. Potential application in breeding of rice varieties with optimized grain morphologies. Slender grain shape.
( Zhao et al., 2018 )
SDN1
CRISPR/Cas
Yangzhou University, China
Increased yield.
( Zhou et al., 2019 )
SDN1
CRISPR/Cas
University of Electronic Science and Technology of China
Xichang University, China
University of Maryland, USA
Promoted rice growth and productivity.
( Miao et al., 2018 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences, China
Purdue University, USA
Increased yield.
( Huang et al., 2018 )
SDN1
CRISPR/Cas
Yunnan University
Chinese Academy of Sciences
BGI-Baoshan, China
Increased grain size and modulated shoot architecture.
( Miao et al., 2020 )
SDN1
CRISPR/Cas
Zhejiang A&
F University
Nanchang University
Chinese Academy of Sciences, China
Purdue University, USA
Dwarf stature and a lesion-mimic phenotype. Fungal resistance: enhanced resistance to the pathogen Magnaporthe oryzae. Increased content of salicylic acid and induced plant defense responses.
(Ma et al., 2018)
SDN1
CRISPR/Cas
Peking University
Chinese Academy of Agricultural Sciences, China
Dwarf and high tillering phenotypes.
( Yang et al., 2017 )
SDN1
CRISPR/Cas
Shenzhen University
The Chinese University of Hong Kong, China
Improved grain yield by promoting outgrowth buds and increasing tiller number.
( Lu et al., 2018 )
SDN1
CRISPR/Cas
Wuhan Institute of Bioengineering
Huazhong Agricultural University, China
Increased rice grain size and yield.
( Wang et al., 2022 )
SDN1
CRISPR/Cas
China National Seed Group Co. Ltd., China
Improved grain length and weight by promoting cell proliferation.
( Wu et al., 2022 )
SDN1
CRISPR/Cas
Chongqing University, China
Longer grains and increased glume cell length.
( Sheng et al., 2022 )
SDN1
CRISPR/Cas
China Agricultural University
Chinese Academy of Sciences, China
Improved grain length and weight by promoting cell proliferation in spikelet hull
( Wu et al., 2022 )
SDN1
CRISPR/Cas
Chongqing University, China
Improved grain quality without severe yield penalty under nitrogen reduction conditions.
( He et al., 2022 )
SDN1
CRISPR/Cas
Rice Research Institute of Shenyang Agricultural University
Tianjin Tianlong Science and Technology Co. LTD.
National Japanica Rice Research and Development Center, China
Enhanced rice grain yield by decoupling panicle number and size
( Song et al., 2022 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences
Shandong Agricultural University
Hainan Yazhou Bay Seed Laboratory, China
Increased grain yield without side effect.
( Gho et al., 2022 )
SDN1
CRISPR/Cas
Kyung Hee University, South Korea
International Rice Research Institute, Philippines
Improved rice yield and immunity.
( Li et al., 2022 )
SDN1
CRISPR/Cas
Huazhong Agricultural University
Chinese Academy of Agricultural Sciences, China

Higher yield than wild-type (WT) plants due to increased grain number per panicle, elevated grain weight, and enhanced harvest index.
( Wei et al., 2022 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences
Shanghai Normal University, China
Increased water use efficiency without growth reductions in well-watered conditions.
( Blankenagel et al., 2022 )
SDN1
CRISPR/Cas
Technical University of Munich
Max Planck Institute of Molecular Plant Physiology
German Research Center for Environmental Health
KWS SAAT SE &
Co.KGaA
Université Technique de Munich
Heinrich Heine University, Germany
LEPSE - Écophysiologie des Plantes sous Stress environnementaux, France

Traits related to storage performance

Improved seed storability. Deterioration of rice grain reduces the quality of rice, resulting in serious economic losses for farmers.
( Ma et al., 2015 )
SDN1
TALENs
China Agricultural University, China

Traits related to industrial utilization

Confer male sterility for hybrid seed production. Male sterility is an important trait, especially for self-pollinated crops such as rice.
( Ma et al., 2019 )
SDN1
CRISPR/Cas
South China Agricultural University, China
Generation of a new thermo-sensitive genic male sterile rice line for hybrid breeding of indica rice.
( Barman et al., 2019 )
SDN1
CRISPR/Cas
China National Rice Research Institute, China
Bangladesh Rice Research Institute, Bangladesh
Fertility restoration of cytoplasmic male sterility.
( Suketomo et al., 2020 )
SDN1
CRISPR/Cas
Tohoku University, Japan
Male sterility and decreased total fatty acid content in the anther.
( Basnet et al., 2019 )
SDN1
CRISPR/Cas
Zhejiang University
Yangtze University, China
Development of commercial thermosensitive genic male sterile lines to accelerate hybrid rice breeding.
( Zhou et al., 2016 )
SDN1
CRISPR/Cas
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources
Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions
South China Agricultural University
China National Hybrid Rice R&
D Center, China
Induction of haploid plants and a reduced seed set for rice breeding.
( Yao et al., 2018 )
SDN2
CRISPR/Cas
ZhongGuanCun Life Science Park, China
Syngenta India Limited
Technology Centre
Medchal Mandal, India
Syngenta Crop Protection
LLC
Research Triangle Park, USA
Asexual propagation trough seeds. Induction of apomeiosis, mitosis instead of meiosis. This proces leads to the production of genetically identical seeds, serving many applications in plant breeding.
( Khanday et al., 2019 )
SDN1
CRISPR/Cas
University of California
Innovative Genomics Institute
Iowa State University, USA
Université Paris-Saclay, France
Genetic variability. The genetically reprogrammed rice plants can act as donor lines to stabilize important agronomic traits or can be a potential resource to create more segregating population.
( K et al., 2021 )
SDN1
CRISPR/Cas
University of Agricultural Sciences
Regional Centre for Biotechnology, India
Creation of photoperiod-/thermo-sensitive genic male-sterile (P/TGMS) lines, important for commercial rice breeding. P/TGMS rice lines are useful germplasm resources for two-line hybrid breeding.
( Lan et al., 2019 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences, China
Generating male sterility lines (MLS). Using MLS in hybrid seed production for monoclinous crops reduces costs and ensures high purity of the varieties because it does not produce pollen and has exserted stigmas.
( Li et al., 2020 )
SDN1
CRISPR/Cas
Peking University Institute of Advanced Agricultural Sciences
School of Advanced Agriculture Sciences and School of Life Sciences
Peking University
Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement,China
Removal of methyl iodide emissions. The release of methyl iodide in the athmospere causes ozone depletion and thus represents an important environmental threat.
( Carlessi et al., 2021 )
SDN1
CRISPR/Cas
PlantLab
Institute of Life Sciences
Scuola Superiore Sant’Anna
University of Pisa
University of Milan, Italy
Enhanced biomass saccharification by altered lignin biosynthesis. The intrinsic recalcitrance of lignocellulose residues requires high energy input for bioethanol production.
( Zhang et al., 2020 )
SDN1
CRISPR/Cas
Huazhong Agricultural University
Hubei University of Arts &
Science
Guangxi University, China
Prolonged basic vegetative growth periods for flexible cropping systems in southern China, as well as in other low-latitude regions. Most of the mid-latitude varities were sensitive to temperature or photoperiod, resulting in low grain yield when cultivated in low-latitude regions.
( Wu et al., 2020 )
SDN1
CRISPR/Cas
Fujian Agricultural and Forestry University
Fujian Academy of Agricultural Sciences
Minjiang University, China
Production of herbicide-sensitive strain to prevent volunteer infestation. Volunteer rice grows when cultivated rice seed fall into fields, overwinter and spontaneously germinate the next spring.
( Komatsu et al., 2020 )

BE
Institute of Agrobiological Sciences
National Agriculture and Food Research Organization (NARO)
Graduate School of Science
Technology and Innovation, Japan
Accelerated domestication of African rice landraces by improving domestication traits such as sheed shattering, lodging and seed yield. The acceleration of the development of high-yield African landrace varieties is important considering that Africa has a strong growing population and prone to food shortage.
( Lacchini et al., 2020 )
SDN1
CRISPR/Cas
University of Milan, Italy
University of Montpellier, France
Wine fermentation: minimize ethyl carbamate (EC) accumulation. EC is a potential carcinogen to humans. EC is mainly produced through the reaction between urea and ethanol during the Chinese wine brewing process.
(Wu et al., 2020)
SDN2
CRISPR/Cas
Jiangnan University
Zhejiang Shuren University, China
Generating male sterility lines (MSL). MS is the absence or non-function of pollen grain in plant or incapability of plants to produce or release functional pollen grains. Using MS lines eliminates the process of mechanical emasculation in hybrid seed production.
( Zou et al., 2017 )
SDN1
CRISPR/Cas
Sichuan Agricultural University, China
Rescued male fertility. Hybrids between divergent populations commonly show hybrid sterility; this reproductive barrier hinders hybrid breeding of the japonica and indica rice subspecies.
( Shen et al., 2017 )
SDN1
CRISPR/Cas
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources
Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions
South China Agricultural University, China
Enabled clonal reproduction trough seeds. Application of the method may enable self-propagation of a broad range of elite F1 hybrid crops.
( Wang et al., 2019 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences, China
Université Paris-Saclay, France
Early maturity of rice varieties. Rice is a tropical short-day plant. The northward cultivation in China is accompanied with daylength extension and temperature decrease, which are unfavorable for rice, to complete flowering and seed setting.
( Li et al., 2017 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences
Jiangsu Academy of Agricultural Sciences, China
Restoring cytoplasmic sterility.
( Kazama et al., 2019 )
SDN2
TALENs
Tohoku University
Tamagawa University
The University of Tokyo
National Institute of Genetics
Tokyo Institute of Technology
Tamagawa University
Japan Science and Technology Agency, Japan
Late flowering time.
( Li et al., 2022 )
SDN1
CRISPR/Cas
Zhejiang University
China Zhejiang Zhengjingyuan Pharmacy Chain Co., Ltd. &
Hangzhou Zhengcaiyuan Pharmaceutical Co., China
Thermosensitive genic male sterile lines with high blast resistance and fragrance quality. Resources for hybrid rice breeding.
( Liang et al., 2022 )
SDN1
CRISPR/Cas
China National Rice Research Institute, China
Enhanced biological nitrogen fixation to reduce the use of inorganic nitrogen fertilizers. Enhanced biofilm formation of soil diazotrophic bacteria by modified root microbiome structure.
( Yan et al., 2022 )
SDN1
CRISPR/Cas
University of California
Bayer Crop Science, USA

Traits related to product color/flavour

Red rice. The pigments of coloured rice contain high levels of proanthocyanidins and anthocyanins which have been recognized as health-promoting nutrients.
( Zhu et al., 2019 )
SDN1
CRISPR/Cas
Xiamen University
Fujian Academy of Agricultural Sciences
Minjiang University, China

Traits related to biotic stress tolerance

Enhanced resistance to insects, no serotonin production and higher salicylic acid levels. Rice brown planthopper (BPH; Nilaparvata lugens Stål) and striped stem borer (SSB; Chilo suppressalis) are the two most serious pests in rice production.
( Lu et al., 2018 )
SDN1
CRISPR/Cas
Zhejiang University
Jiaxing Academy of Agricultural Sciences
Wuxi Hupper Bioseed Ltd.
Hubei Collaborative Innovation Center for Grain Industry, China
Newcastle University, UK
Viral resistance: Partial resistance to rice black-streaked dwarf virus (RBSDV). RBSDV is a serious threat in Chinese rice production.
(Wang et al., 2021)
SDN1
CRISPR/Cas
Jiangsu Academy of Agricultural Sciences
Nanjing Agricultural University, China
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Shan et al., 2013)
SDN1
TALENs
Chinese Academy of Sciences, University of Electronic Science and Technology of China, China
University of Minnesota, USA

Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Zafar et al., 2020)
SDN1
CRISPR/Cas
Constituent College of Pakistan Institute of Engineering and Applied Sciences
University of Information Technology
Engineering and Management Sciences
Constituent College of Pakistan Institute of Engineering and Applied Sciences, Pakistan
Fungal resistance: enhanced resistance to Magnaporthe oryzae, causing rice blast, one of the most destructive diseases affecting rice worldwide.
(Wang et al., 2016)
SDN1
CRISPR/Cas
Chinese Academy of Agriculture, China
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Zhou et al., 2015)
SDN1
CRISPR/Cas
Iowa State University, USA
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Blanvillain-Baufumé et al., 2017)
SDN1
TALENs
IRD-CIRAD-Université, France
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Li et al., 2012)
SDN1
TALENs
Iowa State University, USA
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Wang et al., 2017)
SDN1
TALENs
National University of Singapore, Singapore
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Xie et al., 2017)
SDN1
TALENs
Chinese Academy of Sciences, China
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Zhou et al., 2018)
SDN1
CRISPR/Cas
National Center for Plant Gene Research
Sichuan Agricultural University, China
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Li et al., 2013)
SDN1
TALENs
Iowa State University
Guangxi University, USA
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Cai et al., 2017)
SDN1
TALENs
Shanghai Jiao Tong University
Yunnan Academy of Agricultural Sciences, China
Bacterial and fungal resistance: Resistance to bacterial blight and rice blight. Also spontaneous cell death, altered seed dormancy (pre-harvest sprouting) and enhanced growth.
(Liao et al., 2018)
SDN1
CRISPR/Cas
Sichuan Agricultural University, China
Viral resistance: resistance to rice tungro spherical virus, causing rice tungro disease (RTD). RTD is a serious threat for rice production in tropical Asia.
(Macovei et al., 2018)
SDN1
CRISPR/Cas
International Rice Research Institute (IRRI), Philippines
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease in Southeast Asia and West Africa. Bacteria enter the host and produce a toxin, which prevents the production of chlorophyl.
(Han et al., 2020)
SDN1
TALENs
Chinese Academy of Sciences
Hainan University, China
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease in Southeast Asia and West Africa.
(Wei et al., 2021)
SDN2
CRISPR/Cas
Chinese Academy of Agricultural Sciences, China
Agricultural Research Center, Egypt
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease.
(Li et al., 2020)
SDN1
CRISPR/Cas
College of Life Science and Technology &
College of Horticulture &
Forestry Sciences
Huazhong Agricultural University, China
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Xu et al., 2021)
SDN1
TALENs
Shanghai Jiao Tong University, China
Crop Diseases Research Institute, Pakistan
Bacterial resistance: Strong resistance to Xanthomonas oryzae, causing bacterial blight, a devastating rice disease resulting in yield losses.
(Zeng et al., 2020)
SDN1
CRISPR/Cas
Chinese Academy of Sciences, China
Drought and salt tolerance.
( Kumar et al., 2020 )
SDN1
CRISPR/Cas
ICAR-Indian Agricultural Research Institute
Bhartidasan University, India
Disease resistant thermosensitive genic male sterility (TGMS) with enhanced resistance to rice blast and bacterial blight.
( Li et al., 2019 )
SDN1
CRISPR/Cas
Chinese Academy of Agricultural Sciences, China
Bacterial resistance: Enhanced resistance to both blast and bacterial blight diseases, two major diseases having devastating impact on the yield of rice in most rice-growing countries.
(Zhou et al., 2021)
SDN1
CRISPR/Cas
South China Agricultural University
Huazhong Agricultural University
Yuan Longping High-Tech Agriculture Co. Ltd
Hunan Hybrid Rice Research Center
Yuan Longping High-Tech Agriculture Co. Ltd, China
Bacterial resistance: Resistance/moderately resistance against Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv oryzae (Xoo). BLB is a major constraint in rice production.
(Arulganesh et al., 2022)
SDN1
CRISPR/Cas
Tamil Nadu Agricultural University, India
Viral resistance: resistance to rice tungro disease (RTD), the most important viral disease that limits rice production.
(Kumam et al., 2022)
SDN1
CRISPR/Cas
Tamil Nadu Agricultural University
International Centre for Genetic Engineering and Biotechnology
ICAR-Indian Institute of Rice Research, India
Fungal resistance: Enhanced resistance to blast without affecting the major agronomic traits. Rice blast caused by Magnaporthe oryzae, is a devastating disease affecting rice production globally
(Nawaz et al., 2020)
SDN1
CRISPR/Cas
Guangxi University
South China Agricultural University, China
Fungal resistance: Improved resistance to false smut, caused by Ustilaginoidea virens. False smut is one of the major fungal diseases of rice.
(Liang et al., 2018)
SDN2
CRISPR/Cas
Northwest A&
F University
Fujian Agriculture and Forestry University, China
Bacterial resistance: Enhanced resistance against hemibiotrophic pathogens M. oryzae and Xanthomonas oryzae pv. oryzae (but increased susceptibility to Cochliobolus miyabeanus)
(Kim et al., 2022)
SDN1
CRISPR/Cas
Seoul National University
Kyung Hee University, South Korea
Pennsylvania State University, USA
Eenhanced blast disease resistance
( Liao et al., 2022 )
SDN1
CRISPR/Cas
Sichuan Agricultural University, China
Significant resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo), sheath blight caused by Rhizoctonia solani and rice blast caused by Magnaporthe oryzae.
( Hu et al., 2021 )
SDN1
CRISPR/Cas
Huazhong Agricultural University
Jiangxi Agricultural University
Wuhan Towin Biotechnology Company Limited, China
Broad-spectrum bacterial blight resistance.
( Xu et al., 2019 )
SDN1
CRISPR/Cas
Shanghai Jiao Tong University, China

Traits related to herbicide tolerance

Bispyribac sodium, haloxyfop
( Xu et al., 2021 )

BE
Anhui Academy of Agricultural Sciences, China
Imidazolinone, haloxyfop-R-methyl, glufosinate, dinitroaniline
( Yan et al., 2021 )

BE
Chinese Academy of Agricultural Sciences
China Agricultural University
Ministry of Agriculture and Rural Affairs
Jilin Agricultural University
Zhejiang University
Haloxyfop-R-methyl
( Xu et al., 2020 )

PE
Anhui Academy of Agricultural Science, China
Glyphosate
( Li et al., 2016 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences, China
Bispyribac sodium
( Kuang et al., 2020 )

BE
Chinese Academy of Agricultural Sciences
China Agricultural University
Zhejiang University, China
Norwegian Institute of Bioeconomy Research, Norway
Bispyribac sodium
( Butt et al., 2020 )

PE
King Abdullah University of Science and Technology (KAUST), Saudi Arabia
Herbicide tolerance (ALS-targeting)
( Wang et al., 2020 )
SDN1
CRISPR/Cas
Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement
Yangzhou University
Jiangsu Academy of Agricultural Sciences
Jiangsu University, China
CSIRO Agriculture and Food, Australia
Herbicide tolerance: ALS-inhibiting
(Okuzaki et al., 2004)

ODM
Tohoku University, Japan
Herbicide resistance
( Shimatani et al. 2018 )

BE
Kobe University, Japan
University of Tsukuba, Japan
Imazethapyr, imazapic
( Wang et al., 2020 )
SDN1
CRISPR/Cas
Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement
Yangzhou University
Jiangsu University, China
CSIRO Agriculture and Food, Australia
Glyphosate
( Li et al., 2016 )
SDN1
CRISPR/Cas
Chinese Academy of Sciences, China
Herboxidiene
( Butt et al., 2019 )
SDN1
CRISPR/Cas
King Abdullah University of Science and Technology (KAUST), Saudi Arabia
Universite Paris-Saclay, France
FCD & bipyrazone
( Lu et al., 2021 )
SDN1
CRISPR/Cas
China Agricultural University
Qingdao Kingagroot Compounds Co. Ltd
Guizhou University
Chinese Academy of Sciences, China
Imazamox
( Shimatani et al. 2017 )

BE
Kobe University
University of Tsukuba
Meijo University, Japan
ALS-inhibiting herbicides broad spectrum: Nicosulfuron, imazapic, pyroxsulam, flucarbazone, bispyriba
(Zhang et al., 2020)

BE
Chinese Academy of Sciences
China Agricultural University, China
Haloxyfopo-R-methyl
( Li et al., 2018 )

BE
Chinese Academy of Sciences, China
Dinitroanaline
( Liu et al., 2021 )

BE
Chinese Academy of Agricultural Sciences
China Agricultural University
Zhejiang University
Scientific Observing and Experimental Station of Crop Pests in Guilin, Ministry of Agriculture and Rural Affairs, China
Norwegian Institute of Bioeconomy Research, Norway
Haloxyfop
( Liu et al., 2020 )

BE
Anhui Agricultural University
Anhui Academy of Agricultural Sciences, China
Resistance to ALS-inhibiting herbicides.
( Okuzaki et al., 2003 )

ODM
Tohoku University, Japan
Bispyribac sodium
( Butt et al., 2017 )
SDN2
CRISPR/Cas
King Abdullah University of Science and Technology, Saudi Arabia
Agricultural Research Center, Egypt
Rice University, USA
Improved paraquat resistance in rice without obvious yield penalty.
( Lyu et al., 2022 )
SDN1
CRISPR/Cas
Zhejiang University, China